Thermophile Totally Explained

Everything about Thermophilic totally explained

A thermophile is an organism – a type of extremophile – which thrives at relatively high temperatures,between 40 degrees Celsius (104 degrees Fahrenheit) to 70 degrees Celsius (158 degrees Fahrenheit). Many thermophiles are archaea.
   Thermophiles are found in various geothermally heated regions of the Earth such as hot springs like those in Yellowstone National Park (see image) and deep sea hydrothermal vents, as well as decaying plant matter such as peat bogs and compost.
   As a prerequisite for their survival, thermophiles contain enzymes that can function at high temperature. Some of these enzymes are used in molecular biology (for example heat-stable DNA polymerases for PCR), and in washing agents.
   Thermophiles are classified into obligate and facultative thermophiles: obligate thermophiles (also called extreme thermophiles) require such high temperatures for growth, while facultative thermophiles (also called moderate thermophiles) can thrive at high temperatures but also at lower temperatures (below 50 °C). Hyperthermophiles are particularly extreme thermophiles for which the optimal temperatures are above 80 °C.

Importance of enzymes from thermophiles

The enzymes isolated from some extremophiles have proven to be of great use in the biotechnology industry, able to function under conditions that would denature enzymes taken from most "normal" organisms.
   The most commonly used DNA polymerase for the PCR technique is Taq DNA polymerase, originally isolated from Thermus aquaticus, a bacterial species found in surface aquatic locations such as Yellowstone National Park hot springs. For a few PCR applications, the lack of proofreading by Taq DNA polymerase is a problem.
   The DNA polymerase from Thermococcus litoralis was shown to have a proofreading exonuclease activity. (Mattila et al, 1991)Thermococcus litoralis was isolated from a deep sea hydrothermal vent. This DNA polymerase is marketed as "Vent" polymerase.
   Another heat stable polymerase comes from the organism Pyrococcus furiosus, (Pfu). This organism grows optimally at 100°C, making it a hyperthermophile.
   Taq DNA polymerase is adequate for most PCR, but one study (Hamilton et al, 2001) reported that higher fidelity thermostable DNA polymerases such as Vent account for as much as 30% of DNA polymerase sales.
   In addition, the study of proteins from thermophilic organisms has provided important insight into the mechanism of protein folding because these proteins must be stable at temperatures that would denature typical proteins. Therefore, understanding how thermophilic proteins have evolved to be stable can yield information about the functional modulation of folding landscapes.

Extreme Thermophiles

Thermophiles, meaning heat-loving organisms, are organisms with an optimum growth temperature of 50 °C or more, a maximum of up to 70 °C or more, and a minimum of about 20 °C, but these are only approximate. Some extreme thermophiles (hyperthermophiles) require a very high temperature (80 °C to 105 °C) for growth. Their membranes and proteins are unusually stable at these extremely high temperatures. Thus many important biotechnological processes utilize thermophilic enzymes because of their ability to withstand intense heat.
Many of the hyperthermophiles Archea require elemental sulfur for growth. Some are anaerobes that use the sulfur as an electron acceptor during respiration instead of oxygen. Some are lithotrophs that oxidize sulfur to sulfuric acid as an energy source, thus requiring the microorganism to be adapted to very low pH (for example it's an acidophile as well as thermophile). These organisms are inhabitants of hot, sulfur-rich environments usually associated with volcanism, such as hot springs, geysers and fumaroles. In these places, especially in Yellowstone National Park, we find a zonation of microorganisms according to their temperature optima. Often these organisma are coloured, due to the presence of photosynthetic pigments.

Genomic and Proteomic Features of Thermophiles

The genome and proteome composition of thermophiles are characterized by overrepresentation of purine bases in protein coding sequences, higher GC-content of the structural RNAs, distinct synonymous codon usage, enhanced usage of positively charged residues and aromatic residues, decrease in polar uncharged residues in the encoded protein (Das et al, 2006)

Books Examining Thermophiles

"Microbes of Yellowstone

" by Sheehan Kathy B, Patterson David J, Dicks Brett Leigh, and Henson Joan M. The Globe Pequot Press (2006) Microorganisms and Life at High Temperatures by Brock Thomas D. Springer-Verlag (1978).

Further Information

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